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Proteintech anti kras
Anti Kras, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/18295+1+ap/HRAS-Specific+Antibody/pm41903755-382-63-64
Average 95 stars, based on 61 article reviews
anti kras - by Bioz Stars, 2026-09
95/100 stars

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Related Articles

Binding Assay:

Article Title: The ERBB network facilitates KRAS-driven lung tumorigenesis.
Article Snippet: For immunoblotting, cells were lysed in RIPAHi buffer [150 mM NaCl, 50 mM tris (pH 7.5), 1% NP-40, 0.5% sodium deoxycholic acid, 1% SDS plus Complete protease/phosphatase inhibitor cocktails (Sigma-Aldrich)] and, after Western blotting, probed with the following primary antibodies: p-ERK Thr202;Tyr204 (E-4) (SC7383, Santa Cruz Biotechnology), p-EGFR Tyr1068 (3777, Cell Signaling Technology), p-ERBB2 Tyr1248 (06-229, Millipore), p-ERBB3 Tyr1197 (4561, Cell Signaling Technology), ERK1/2 (4695, Cell Signaling Technology), EGFR (06-847, Millipore), ERBB2 (OP15L, Merck), and ERBB3 (05-390, Millipore). .. RAS:RAF binding assays were performed using a commercial RAS activity assay kit (Cytoskeleton) and probed with pan-RAS (AESA02, Cytoskeleton), KRAS (SC-30, Santa Cruz Biotechnology), or HRAS-specific (18295-1-AP, Proteintech) antibodies (41). ..

Article Title: The ERBB network facilitates KRAS-driven lung tumorigenesis
Article Snippet: For immunoblotting, cells were lysed in RIPA Hi buffer (150 mM NaCl, 50 mM Tris [pH 7.5], 1% NP-40, 0.5% sodium deoxycholic acid, 1% SDS plus Complete protease/phosphatase inhibitor cocktails [Sigma-Aldrich]) and after western blotting, probed with the following primary antibodies: p-ERK Thr202;Tyr204 (E-4), Santa Cruz SC7383; p-EGFR Tyr1068, Cell Signaling 3777; p-ERBB2 Tyr 1248, Millipore 06-229; p-ERBB3 Tyr 1197 Cell Signaling 4561; ERK1/2 Cell Signaling 4695; EGFR Millipore 06-847; ERBB2 Merck OP15L; ERBB3 Millipore 05-390. .. RAS:RAF binding assays were performed using a commercial RAS activity assay kit (Cytoskeleton) and probed with pan-RAS (AESA02, Cytoskeleton), KRAS (SC-30, Santa Cruz), or HRAS-specific (18295-1-AP, Proteintech) antibodies [ 41 ]. .. All genotyping was subsequently performed by Transnetyx Inc.

Activity Assay:

Article Title: The ERBB network facilitates KRAS-driven lung tumorigenesis.
Article Snippet: For immunoblotting, cells were lysed in RIPAHi buffer [150 mM NaCl, 50 mM tris (pH 7.5), 1% NP-40, 0.5% sodium deoxycholic acid, 1% SDS plus Complete protease/phosphatase inhibitor cocktails (Sigma-Aldrich)] and, after Western blotting, probed with the following primary antibodies: p-ERK Thr202;Tyr204 (E-4) (SC7383, Santa Cruz Biotechnology), p-EGFR Tyr1068 (3777, Cell Signaling Technology), p-ERBB2 Tyr1248 (06-229, Millipore), p-ERBB3 Tyr1197 (4561, Cell Signaling Technology), ERK1/2 (4695, Cell Signaling Technology), EGFR (06-847, Millipore), ERBB2 (OP15L, Merck), and ERBB3 (05-390, Millipore). .. RAS:RAF binding assays were performed using a commercial RAS activity assay kit (Cytoskeleton) and probed with pan-RAS (AESA02, Cytoskeleton), KRAS (SC-30, Santa Cruz Biotechnology), or HRAS-specific (18295-1-AP, Proteintech) antibodies (41). ..

Article Title: The ERBB network facilitates KRAS-driven lung tumorigenesis
Article Snippet: For immunoblotting, cells were lysed in RIPA Hi buffer (150 mM NaCl, 50 mM Tris [pH 7.5], 1% NP-40, 0.5% sodium deoxycholic acid, 1% SDS plus Complete protease/phosphatase inhibitor cocktails [Sigma-Aldrich]) and after western blotting, probed with the following primary antibodies: p-ERK Thr202;Tyr204 (E-4), Santa Cruz SC7383; p-EGFR Tyr1068, Cell Signaling 3777; p-ERBB2 Tyr 1248, Millipore 06-229; p-ERBB3 Tyr 1197 Cell Signaling 4561; ERK1/2 Cell Signaling 4695; EGFR Millipore 06-847; ERBB2 Merck OP15L; ERBB3 Millipore 05-390. .. RAS:RAF binding assays were performed using a commercial RAS activity assay kit (Cytoskeleton) and probed with pan-RAS (AESA02, Cytoskeleton), KRAS (SC-30, Santa Cruz), or HRAS-specific (18295-1-AP, Proteintech) antibodies [ 41 ]. .. All genotyping was subsequently performed by Transnetyx Inc.

Western Blot:

Article Title: Protein farnesylation is upregulated in Alzheimer’s human brains and neuron-specific suppression of farnesyltransferase mitigates pathogenic processes in Alzheimer’s model mice
Article Snippet: H-Ras (C20) , Santa Cruz , sc-520 , Rabbit/IgG , 21 kD , WB. .. H-Ras , ProteinTech , 18295–1-AP , Rabbit/IgG , 21 kD , WB. .. HDJ2 (KA2A5.6) , Invitrogen , MA5-12748 , Mouse/IgG1 , 40 kD , WB.

Article Title: Evaluation of the selectivity and sensitivity of isoform- and mutation-specific RAS antibodies
Article Snippet: H , SC-520 , L3015 , Santa Cruz Biol. , polyclonal , n/a , Rabbit , H, M, R , WB, IP, IF, ELISA , Human HRAS C-terminal peptide. .. H , 18295–1-AP , 22367 , Proteintech , polyclonal , n/a , Rabbit , H, M , WB, ELISA, IF, IHC, FC , Peptide. ..

Virus:

Article Title: H-Ras induces exuberant de novo dendritic protrusion growth in mature neurons regardless of cell type.
Article Snippet: .. Antibodies Guinea pig anti-Bassoon antibody Synaptic Systems Cat# 141 004; RRID: AB_2290619 Mouse anti-Parvalbumin antibody Swant Cat#. 235; RRID: AB_10000343 Rabbit anti-Homer1 antibody Synaptic Systems Cat#. 160 003; RRID: AB_887730 Rabbit anti-H-Ras antibody Proteintech Cat#. 18295-1-AP; RRID: AB_2121046 Goat Anti-Guinea pig IgG H&L Alexa Fluor 405 Abcam Cat# ab175678; RRID: AB_2827755 Goat anti-Mouse IgG H + L Alexa Fluor 633 ThermoFisher Cat# A-21052; RRID: AB_2535719 Goat anti-Rabbit IgG H + L Alexa Fluor 633 ThermoFisher Cat# A-21071; RRID: AB_2535732 Bacterial and virus strains AAV-CAG-B3-RafRBD-2A-GA-HRas This paper Upon request AAV-CAG-DIO-ddFPB-RafRBD-2AddGFPA-HRas This paper Upon request AAV-CAG-DIO-ddFPB-RafRBD-2A-ddGFPA This paper Upon request AAV-CAG-DIO-ddFPB-2A-ddGFPA This paper Upon request AAV-CAG-tdTomato Edward Boyden RRID: Addgene_59462 AAV1-Camk2-0.4-Cre-SV40 James M. Wilson Lab RRID: Addgene_105558 AAV9-CAG-Flex-tdTomato Edward Boyden RRID: Addgene_28306 Chemicals, peptides, and recombinant proteins Adenosine triphosphate (ATP) Sigma-Aldrich Cat# A2383-1G Ascorbic Acid Sigma-Aldrich Cat# A92902 Bicuculine Enzo Cat# BML-EA149-005 Calcium chloride (CaCl) Honeywell Fluka Cat# 21114-1L Cesium hydroxide Sigma-Aldrich Cat# 232041-50G DAPI Fluoromount-G SouthernBiotech Cat# 0100-20 D-APV Sigma-Aldrich Cat# A528 D-Glucose Sigma-Aldrich Cat# G7021 EGTA Sigma-Aldrich Cat# E0396-10G Gluconic acid Sigma-Aldrich Cat# G1951 Guanosine triphosphate (GTP) Sigma-Aldrich Cat# G8877-25MG HEPES Sigma-Aldrich Cat# H3375 Horse serum Thermo Fisher Cat# 26050-088 Isoflurane MWI Cat# 07-890-8115 L-Glutamine Thermo Fisher Cat# 25030081 Lidocaine N-ethyl bromide Sigma-Aldrich Cat# L5783 Magnesium chloride (MgCl) Honeywell Fluka Cat# 63020-1L Magnesium sulfate (MgSO4) Sigma-Aldrich Cat# M3409-10X1ML Meloxicam Bio-Serv Cat# MD275-0125 MEM Eagle medium Thermo Fisher Cat# 11965092 MNI glutamate Tocris Cat# 1490 Normal goat serum Thermo Fisher Cat# 50062Z Paraformaldehyde (PFA) Thermo Fisher Cat# J19943.K2 (Continued on next page) 18 iScience 27, 110535, August 16, 2024 .. Phosphate-buffered saline (PBS) Thermo Fisher Cat.# 10010049 Phosphocreatine Sigma-Aldrich Cat# P7936-1G Potassium chloride (KCl) Sigma-Aldrich Cat# 1603311000 Sodium bicarbonate (NaHCO3) Sigma-Aldrich Cat# S6014 Sodium phosphate monobasic monohydrate (NaH2PO4) Sigma-Aldrich Cat# S5011

Bioprocessing:

Article Title: H-Ras induces exuberant de novo dendritic protrusion growth in mature neurons regardless of cell type.
Article Snippet: .. Antibodies Guinea pig anti-Bassoon antibody Synaptic Systems Cat# 141 004; RRID: AB_2290619 Mouse anti-Parvalbumin antibody Swant Cat#. 235; RRID: AB_10000343 Rabbit anti-Homer1 antibody Synaptic Systems Cat#. 160 003; RRID: AB_887730 Rabbit anti-H-Ras antibody Proteintech Cat#. 18295-1-AP; RRID: AB_2121046 Goat Anti-Guinea pig IgG H&L Alexa Fluor 405 Abcam Cat# ab175678; RRID: AB_2827755 Goat anti-Mouse IgG H + L Alexa Fluor 633 ThermoFisher Cat# A-21052; RRID: AB_2535719 Goat anti-Rabbit IgG H + L Alexa Fluor 633 ThermoFisher Cat# A-21071; RRID: AB_2535732 Bacterial and virus strains AAV-CAG-B3-RafRBD-2A-GA-HRas This paper Upon request AAV-CAG-DIO-ddFPB-RafRBD-2AddGFPA-HRas This paper Upon request AAV-CAG-DIO-ddFPB-RafRBD-2A-ddGFPA This paper Upon request AAV-CAG-DIO-ddFPB-2A-ddGFPA This paper Upon request AAV-CAG-tdTomato Edward Boyden RRID: Addgene_59462 AAV1-Camk2-0.4-Cre-SV40 James M. Wilson Lab RRID: Addgene_105558 AAV9-CAG-Flex-tdTomato Edward Boyden RRID: Addgene_28306 Chemicals, peptides, and recombinant proteins Adenosine triphosphate (ATP) Sigma-Aldrich Cat# A2383-1G Ascorbic Acid Sigma-Aldrich Cat# A92902 Bicuculine Enzo Cat# BML-EA149-005 Calcium chloride (CaCl) Honeywell Fluka Cat# 21114-1L Cesium hydroxide Sigma-Aldrich Cat# 232041-50G DAPI Fluoromount-G SouthernBiotech Cat# 0100-20 D-APV Sigma-Aldrich Cat# A528 D-Glucose Sigma-Aldrich Cat# G7021 EGTA Sigma-Aldrich Cat# E0396-10G Gluconic acid Sigma-Aldrich Cat# G1951 Guanosine triphosphate (GTP) Sigma-Aldrich Cat# G8877-25MG HEPES Sigma-Aldrich Cat# H3375 Horse serum Thermo Fisher Cat# 26050-088 Isoflurane MWI Cat# 07-890-8115 L-Glutamine Thermo Fisher Cat# 25030081 Lidocaine N-ethyl bromide Sigma-Aldrich Cat# L5783 Magnesium chloride (MgCl) Honeywell Fluka Cat# 63020-1L Magnesium sulfate (MgSO4) Sigma-Aldrich Cat# M3409-10X1ML Meloxicam Bio-Serv Cat# MD275-0125 MEM Eagle medium Thermo Fisher Cat# 11965092 MNI glutamate Tocris Cat# 1490 Normal goat serum Thermo Fisher Cat# 50062Z Paraformaldehyde (PFA) Thermo Fisher Cat# J19943.K2 (Continued on next page) 18 iScience 27, 110535, August 16, 2024 .. Phosphate-buffered saline (PBS) Thermo Fisher Cat.# 10010049 Phosphocreatine Sigma-Aldrich Cat# P7936-1G Potassium chloride (KCl) Sigma-Aldrich Cat# 1603311000 Sodium bicarbonate (NaHCO3) Sigma-Aldrich Cat# S6014 Sodium phosphate monobasic monohydrate (NaH2PO4) Sigma-Aldrich Cat# S5011

Recombinant:

Article Title: H-Ras induces exuberant de novo dendritic protrusion growth in mature neurons regardless of cell type.
Article Snippet: .. Antibodies Guinea pig anti-Bassoon antibody Synaptic Systems Cat# 141 004; RRID: AB_2290619 Mouse anti-Parvalbumin antibody Swant Cat#. 235; RRID: AB_10000343 Rabbit anti-Homer1 antibody Synaptic Systems Cat#. 160 003; RRID: AB_887730 Rabbit anti-H-Ras antibody Proteintech Cat#. 18295-1-AP; RRID: AB_2121046 Goat Anti-Guinea pig IgG H&L Alexa Fluor 405 Abcam Cat# ab175678; RRID: AB_2827755 Goat anti-Mouse IgG H + L Alexa Fluor 633 ThermoFisher Cat# A-21052; RRID: AB_2535719 Goat anti-Rabbit IgG H + L Alexa Fluor 633 ThermoFisher Cat# A-21071; RRID: AB_2535732 Bacterial and virus strains AAV-CAG-B3-RafRBD-2A-GA-HRas This paper Upon request AAV-CAG-DIO-ddFPB-RafRBD-2AddGFPA-HRas This paper Upon request AAV-CAG-DIO-ddFPB-RafRBD-2A-ddGFPA This paper Upon request AAV-CAG-DIO-ddFPB-2A-ddGFPA This paper Upon request AAV-CAG-tdTomato Edward Boyden RRID: Addgene_59462 AAV1-Camk2-0.4-Cre-SV40 James M. Wilson Lab RRID: Addgene_105558 AAV9-CAG-Flex-tdTomato Edward Boyden RRID: Addgene_28306 Chemicals, peptides, and recombinant proteins Adenosine triphosphate (ATP) Sigma-Aldrich Cat# A2383-1G Ascorbic Acid Sigma-Aldrich Cat# A92902 Bicuculine Enzo Cat# BML-EA149-005 Calcium chloride (CaCl) Honeywell Fluka Cat# 21114-1L Cesium hydroxide Sigma-Aldrich Cat# 232041-50G DAPI Fluoromount-G SouthernBiotech Cat# 0100-20 D-APV Sigma-Aldrich Cat# A528 D-Glucose Sigma-Aldrich Cat# G7021 EGTA Sigma-Aldrich Cat# E0396-10G Gluconic acid Sigma-Aldrich Cat# G1951 Guanosine triphosphate (GTP) Sigma-Aldrich Cat# G8877-25MG HEPES Sigma-Aldrich Cat# H3375 Horse serum Thermo Fisher Cat# 26050-088 Isoflurane MWI Cat# 07-890-8115 L-Glutamine Thermo Fisher Cat# 25030081 Lidocaine N-ethyl bromide Sigma-Aldrich Cat# L5783 Magnesium chloride (MgCl) Honeywell Fluka Cat# 63020-1L Magnesium sulfate (MgSO4) Sigma-Aldrich Cat# M3409-10X1ML Meloxicam Bio-Serv Cat# MD275-0125 MEM Eagle medium Thermo Fisher Cat# 11965092 MNI glutamate Tocris Cat# 1490 Normal goat serum Thermo Fisher Cat# 50062Z Paraformaldehyde (PFA) Thermo Fisher Cat# J19943.K2 (Continued on next page) 18 iScience 27, 110535, August 16, 2024 .. Phosphate-buffered saline (PBS) Thermo Fisher Cat.# 10010049 Phosphocreatine Sigma-Aldrich Cat# P7936-1G Potassium chloride (KCl) Sigma-Aldrich Cat# 1603311000 Sodium bicarbonate (NaHCO3) Sigma-Aldrich Cat# S6014 Sodium phosphate monobasic monohydrate (NaH2PO4) Sigma-Aldrich Cat# S5011

Enzyme-linked Immunosorbent Assay:

Article Title: Evaluation of the selectivity and sensitivity of isoform- and mutation-specific RAS antibodies
Article Snippet: H , SC-520 , L3015 , Santa Cruz Biol. , polyclonal , n/a , Rabbit , H, M, R , WB, IP, IF, ELISA , Human HRAS C-terminal peptide. .. H , 18295–1-AP , 22367 , Proteintech , polyclonal , n/a , Rabbit , H, M , WB, ELISA, IF, IHC, FC , Peptide. ..

Immunohistochemistry:

Article Title: Evaluation of the selectivity and sensitivity of isoform- and mutation-specific RAS antibodies
Article Snippet: H , SC-520 , L3015 , Santa Cruz Biol. , polyclonal , n/a , Rabbit , H, M, R , WB, IP, IF, ELISA , Human HRAS C-terminal peptide. .. H , 18295–1-AP , 22367 , Proteintech , polyclonal , n/a , Rabbit , H, M , WB, ELISA, IF, IHC, FC , Peptide. ..



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